The tennis star first had his visa cancelled on arrival in Melbourne last week when his COVID vaccination exemption was questioned.

But he won a court appeal against the cancellation that allowed him to remain in the country.

At the time, the Australian government said it would continue to look at whether he could stay, which was a decision that was entirely at the discretion of immigration minister Alex Hawke.

Djokovic's lawyers have now asked the court at a preliminary hearing for an injunction to block his deportation from Australia.

Immigration minister's statement

Mr Hawke said he made his judgement after "carefully" considering information from the Department of Home Affairs, the Australian Border Force and Mr Djokovic.

"Today I exercised my power under section 133C(3) of the Migration Act to cancel the visa held by Mr Novak Djokovic on health and good order grounds, on the basis that it was in the public interest to do so," he said in a statement.

"The Morrison government is firmly committed to protecting Australia's borders, particularly in relation to the COVID-19 pandemic," he added, referring to Prime Minister Scott Morrison.

Djokovic vs Australia: What both sides have said until now

Under the section of the Migration Act used by the minister, Djokovic will not be able to secure a visa to come to Australia for three years, except in compelling circumstances that affect the country's interest.

Commenting on the decision, Mr Morrison said Australians have made "many sacrifices" during the pandemic.

"They rightly expect the result of those sacrifices to be protected," he added. "The pandemic has been incredibly difficult for every Australian, but we have stuck together and saved lives and livelihoods.

"Our strong border protection policies have kept Australians safe, prior to COVID and now during the pandemic."

What are Australia's travel rules?

What about the Australian Open?

The second cancellation of Djokovic's visa comes after he was drawn against fellow Serb Miomir Kecmanovic in the Australian Open, which is due to begin on Monday.

The men's tennis world number one could still file a legal challenge, but if not, his hopes of winning a 10th title at Melbourne Park and 21st grand slam crown will come to an end.

In order to stand a chance at competing, his lawyers would need to go before a judge of the Federal Circuit and Family Court or a higher judge of the Federal Court to get two urgent orders.

One order would be an injunction to prevent his deportation, like the one he gained last week.

The second would order Mr Hawke to grant Djokovic a visa to play.

If he is forced out of the tournament ahead of Monday's order of play being announced, the seeds will be shuffled around, with fifth seed Andrey Rublev taking the tennis star's place.

The declaration form problem and the French magazine interview

Djokovic's case was not helped after he admitted his declaration form to enter Australia falsely claimed he had not travelled in the two weeks prior to his trip, which he attributed to a mistake by his agent.

He also confessed to meeting a journalist for a face-to-face interview and photoshoot for French magazine L'Equipe in Belgrade after testing positive for COVID.